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What are the thermal cycler settings required for the TruSight Oncology Comprehensive workflow?

1Answers
SadieFitz
12/17/2025, 01:12:39 PM

Setting up your thermal cyclers correctly is crucial for a successful TruSight Oncology Comprehensive assay. First, program the thermal cyclers for pre-amplification. Refer to the TruSight Oncology Comprehensive workflow manual for detailed instructions on programming the thermal cyclers for the library preparation and enrichment steps. Ensure the programs are saved and ready. Next, set up the post-amplification thermal cyclers. Again, follow the manual's guidance to program these cyclers correctly. Accurate programming of both pre- and post-amplification thermal cyclers, as specified in the TruSight Oncology Comprehensive workflow protocol, will help ensure accurate and reliable results.

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