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How do I calibrate a Sper Scientific 300069 Clinical Refractometer?

1Answers
IslaFitz
12/17/2025, 02:26:53 PM

Calibrating your Sper Scientific 300069 Clinical Refractometer is crucial for ensuring accurate measurements. Here's a straightforward guide to help you calibrate your device effectively:

  1. Zero Point Calibration:

    • Start by opening the COVER PLATE.
    • Apply a few drops of distilled water (provided with your device) onto the PRISM platform.
    • Close the COVER PLATE.
    • Adjust the CALIBRATION screw until the boundary between the light and dark areas aligns with the bottom line on the scale.
  2. Preparing for Testing:

    • Carefully dry both the prism platform and the COVER PLATE to remove any residual moisture.
    • Place a few drops of your test solution onto the prism and close the COVER PLATE. This will allow the solution to spread evenly.
  3. Testing Procedure:

    • Point the front of the refractometer towards a light source and focus the eyepiece on the boundary line.
    • The position of this boundary line on the scale directly indicates the v/v percentage of alcohol in your sample at 20°C.
  4. Post-Testing Care:

    • After each use, clean the prism with distilled water and a soft cloth to remove any remaining residue. This prevents contamination and maintains accuracy.
    • Remember that the temperature of the zero-point calibration should match the temperature of your test solution. Adjust the zero point every 30 minutes if there are significant temperature variations.

Additional Tips:

  • Avoid submerging the unit or allowing any liquid to enter the main body of the refractometer.
  • Clean the refractometer after each use with a soft cotton cloth to avoid scratching the prism surface and ensure longevity.
  • Store the device in a clean, dry, and non-corrosive environment, protecting it from strong impacts. Properly storing your Sper Scientific 300069 will help to maintain the integrity of its components.
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