The Difference Between Coomassie Brilliant Blue G250 and R250

1 Answers
DiGabrielle
07/30/25 6:30am
The differences between Coomassie Brilliant Blue G250 and R250 are as follows: Differences in Usage: Coomassie Brilliant Blue R250 binds to proteins through van der Waals bonds, with a staining sensitivity five times higher than Amido Black, making it suitable for SDS electrophoresis microprotein staining. Coomassie Brilliant Blue G250 is insoluble in trichloroacetic acid and forms a colloid, selectively staining proteins with almost no background color. Therefore, it is often used for staining requiring good reproducibility and stability, making it suitable for quantitative analysis. Different Parameters: Coomassie Brilliant Blue G250 has two more methyl groups than Coomassie Brilliant Blue R-250.
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